YPR153W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 5.0%
Arg[R] 3 2.1%
Asn[N] 6 4.3%
Asp[D] 3 2.1%
Cys[C] 2 1.4%
Gln[Q] 2 1.4%
Glu[E] 0 0.0%
Gly[G] 12 8.6%
His[H] 1 0.7%
Ile[I] 9 6.4%
Leu[L] 12 8.6%
Lys[K] 5 3.6%
Met[M] 6 4.3%
Phe[F] 10 7.1%
Pro[P] 5 3.6%
Ser[S] 13 9.3%
Thr[T] 10 7.1%
Trp[W] 5 3.6%
Tyr[Y] 12 8.6%
Val[V] 17 12.1%
Total Length 140

Instability index
Instability index (II) 18.43
This classifies the protein as stable

Atomic composition
Carbon 749
Hydrogen 1109
Nitrogen 169
Oxygen 190
Sulfur 8
Total number of atoms 2225
Formula = C749H1109N169O190S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 45505 M-1 cm-1
assuming NO Cys residues appear as half cystines 45380 M-1 cm-1
assuming all Cys residues are reduced 45380 M-1 cm-1
assuming all pairs of Cys residues form cystines 45505 M-1 cm-1

Aliphatic index
Aliphatic index 98.71

Coding Region Translation Calculations
Codon Bias 0.147
Codon Adaptation Index 0.141
Frequency of Optimal Codons 0.496
Hydropathicity of Protein 0.639
Aromaticity Score 0.193