Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
24 |
3.3% |
| Arg[R] |
33 |
4.5% |
| Asn[N] |
61 |
8.4% |
| Asp[D] |
28 |
3.8% |
| Cys[C] |
5 |
0.7% |
| Gln[Q] |
31 |
4.3% |
| Glu[E] |
76 |
10.4% |
| Gly[G] |
26 |
3.6% |
| His[H] |
21 |
2.9% |
| Ile[I] |
52 |
7.1% |
| Leu[L] |
76 |
10.4% |
| Lys[K] |
80 |
11.0% |
| Met[M] |
15 |
2.1% |
| Phe[F] |
20 |
2.7% |
| Pro[P] |
17 |
2.3% |
| Ser[S] |
58 |
8.0% |
| Thr[T] |
56 |
7.7% |
| Trp[W] |
4 |
0.5% |
| Tyr[Y] |
15 |
2.1% |
| Val[V] |
31 |
4.3% |
| Total Length |
729 |
|
| Instability index (II) |
45.95 |
| This classifies the protein as unstable |
|
| Carbon |
3674 |
| Hydrogen |
5992 |
| Nitrogen |
1046 |
| Oxygen |
1159 |
| Sulfur |
20 |
| Total number of atoms |
11891 |
| Formula = C3674H5992N1046O1159S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
44600 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
44350 M-1 cm-1 |
| assuming all Cys residues are reduced |
44350 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
44600 M-1 cm-1 |
|
|
| Codon Bias |
-0.044 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.399 |
| Hydropathicity of Protein |
-0.778 |
| Aromaticity Score |
0.053 |
|
