THI22/YPR121W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 42 7.3%
Arg[R] 21 3.7%
Asn[N] 24 4.2%
Asp[D] 34 5.9%
Cys[C] 22 3.8%
Gln[Q] 16 2.8%
Glu[E] 36 6.3%
Gly[G] 35 6.1%
His[H] 11 1.9%
Ile[I] 43 7.5%
Leu[L] 53 9.3%
Lys[K] 38 6.6%
Met[M] 12 2.1%
Phe[F] 18 3.1%
Pro[P] 27 4.7%
Ser[S] 33 5.8%
Thr[T] 37 6.5%
Trp[W] 4 0.7%
Tyr[Y] 22 3.8%
Val[V] 44 7.7%
Total Length 572

Instability index
Instability index (II) 35.51
This classifies the protein as stable

Atomic composition
Carbon 2816
Hydrogen 4479
Nitrogen 739
Oxygen 845
Sulfur 34
Total number of atoms 8913
Formula = C2816H4479N739O845S34

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 56155 M-1 cm-1
assuming NO Cys residues appear as half cystines 54780 M-1 cm-1
assuming all Cys residues are reduced 54780 M-1 cm-1
assuming all pairs of Cys residues form cystines 56155 M-1 cm-1

Aliphatic index
Aliphatic index 95.10

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000