FHL1/YPR104C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 68 7.3%
Arg[R] 37 4.0%
Asn[N] 70 7.5%
Asp[D] 61 6.5%
Cys[C] 2 0.2%
Gln[Q] 52 5.6%
Glu[E] 78 8.3%
Gly[G] 35 3.7%
His[H] 19 2.0%
Ile[I] 54 5.8%
Leu[L] 58 6.2%
Lys[K] 82 8.8%
Met[M] 11 1.2%
Phe[F] 22 2.4%
Pro[P] 53 5.7%
Ser[S] 103 11.0%
Thr[T] 67 7.2%
Trp[W] 3 0.3%
Tyr[Y] 15 1.6%
Val[V] 46 4.9%
Total Length 936

Instability index
Instability index (II) 46.80
This classifies the protein as unstable

Atomic composition
Carbon 4447
Hydrogen 7168
Nitrogen 1292
Oxygen 1522
Sulfur 13
Total number of atoms 14442
Formula = C4447H7168N1292O1522S13

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 38975 M-1 cm-1
assuming NO Cys residues appear as half cystines 38850 M-1 cm-1
assuming all Cys residues are reduced 38850 M-1 cm-1
assuming all pairs of Cys residues form cystines 38975 M-1 cm-1

Aliphatic index
Aliphatic index 68.18

Coding Region Translation Calculations
Codon Bias -0.030
Codon Adaptation Index 0.106
Frequency of Optimal Codons 0.397
Hydropathicity of Protein -0.902
Aromaticity Score 0.043