Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
18 |
3.9% |
| Arg[R] |
19 |
4.2% |
| Asn[N] |
32 |
7.0% |
| Asp[D] |
25 |
5.5% |
| Cys[C] |
4 |
0.9% |
| Gln[Q] |
12 |
2.6% |
| Glu[E] |
27 |
5.9% |
| Gly[G] |
22 |
4.8% |
| His[H] |
12 |
2.6% |
| Ile[I] |
30 |
6.6% |
| Leu[L] |
50 |
11.0% |
| Lys[K] |
31 |
6.8% |
| Met[M] |
8 |
1.8% |
| Phe[F] |
13 |
2.9% |
| Pro[P] |
28 |
6.1% |
| Ser[S] |
58 |
12.7% |
| Thr[T] |
18 |
3.9% |
| Trp[W] |
5 |
1.1% |
| Tyr[Y] |
13 |
2.9% |
| Val[V] |
31 |
6.8% |
| Total Length |
456 |
|
| Instability index (II) |
54.78 |
| This classifies the protein as unstable |
|
| Carbon |
2255 |
| Hydrogen |
3591 |
| Nitrogen |
617 |
| Oxygen |
694 |
| Sulfur |
12 |
| Total number of atoms |
7169 |
| Formula = C2255H3591N617O694S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
47120 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
46870 M-1 cm-1 |
| assuming all Cys residues are reduced |
46870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
47120 M-1 cm-1 |
|
|
| Codon Bias |
0.049 |
| Codon Adaptation Index |
0.135 |
| Frequency of Optimal Codons |
0.429 |
| Hydropathicity of Protein |
-0.363 |
| Aromaticity Score |
0.068 |
|
