Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
68 |
10.0% |
| Arg[R] |
22 |
3.2% |
| Asn[N] |
31 |
4.6% |
| Asp[D] |
34 |
5.0% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
23 |
3.4% |
| Glu[E] |
35 |
5.1% |
| Gly[G] |
58 |
8.5% |
| His[H] |
17 |
2.5% |
| Ile[I] |
36 |
5.3% |
| Leu[L] |
63 |
9.3% |
| Lys[K] |
43 |
6.3% |
| Met[M] |
11 |
1.6% |
| Phe[F] |
28 |
4.1% |
| Pro[P] |
38 |
5.6% |
| Ser[S] |
57 |
8.4% |
| Thr[T] |
37 |
5.4% |
| Trp[W] |
9 |
1.3% |
| Tyr[Y] |
26 |
3.8% |
| Val[V] |
42 |
6.2% |
| Total Length |
680 |
|
| Instability index (II) |
31.18 |
| This classifies the protein as stable |
|
| Carbon |
3321 |
| Hydrogen |
5162 |
| Nitrogen |
886 |
| Oxygen |
993 |
| Sulfur |
13 |
| Total number of atoms |
10375 |
| Formula = C3321H5162N886O993S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
88365 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
88240 M-1 cm-1 |
| assuming all Cys residues are reduced |
88240 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
88365 M-1 cm-1 |
|
|
| Codon Bias |
0.590 |
| Codon Adaptation Index |
0.489 |
| Frequency of Optimal Codons |
0.750 |
| Hydropathicity of Protein |
-0.212 |
| Aromaticity Score |
0.093 |
|
