Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
19 |
3.4% |
| Arg[R] |
16 |
2.8% |
| Asn[N] |
48 |
8.5% |
| Asp[D] |
44 |
7.8% |
| Cys[C] |
7 |
1.2% |
| Gln[Q] |
21 |
3.7% |
| Glu[E] |
44 |
7.8% |
| Gly[G] |
19 |
3.4% |
| His[H] |
10 |
1.8% |
| Ile[I] |
44 |
7.8% |
| Leu[L] |
49 |
8.7% |
| Lys[K] |
38 |
6.7% |
| Met[M] |
9 |
1.6% |
| Phe[F] |
26 |
4.6% |
| Pro[P] |
16 |
2.8% |
| Ser[S] |
75 |
13.3% |
| Thr[T] |
31 |
5.5% |
| Trp[W] |
5 |
0.9% |
| Tyr[Y] |
20 |
3.5% |
| Val[V] |
25 |
4.4% |
| Total Length |
566 |
|
| Instability index (II) |
47.92 |
| This classifies the protein as unstable |
|
| Carbon |
2819 |
| Hydrogen |
4388 |
| Nitrogen |
746 |
| Oxygen |
938 |
| Sulfur |
16 |
| Total number of atoms |
8907 |
| Formula = C2819H4388N746O938S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
57675 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
57300 M-1 cm-1 |
| assuming all Cys residues are reduced |
57300 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
57675 M-1 cm-1 |
|
|
| Codon Bias |
-0.005 |
| Codon Adaptation Index |
0.136 |
| Frequency of Optimal Codons |
0.424 |
| Hydropathicity of Protein |
-0.559 |
| Aromaticity Score |
0.090 |
|
