Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
44 |
4.1% |
| Arg[R] |
45 |
4.2% |
| Asn[N] |
144 |
13.4% |
| Asp[D] |
42 |
3.9% |
| Cys[C] |
12 |
1.1% |
| Gln[Q] |
64 |
6.0% |
| Glu[E] |
34 |
3.2% |
| Gly[G] |
43 |
4.0% |
| His[H] |
19 |
1.8% |
| Ile[I] |
60 |
5.6% |
| Leu[L] |
110 |
10.2% |
| Lys[K] |
44 |
4.1% |
| Met[M] |
12 |
1.1% |
| Phe[F] |
32 |
3.0% |
| Pro[P] |
65 |
6.0% |
| Ser[S] |
127 |
11.8% |
| Thr[T] |
78 |
7.3% |
| Trp[W] |
7 |
0.7% |
| Tyr[Y] |
35 |
3.3% |
| Val[V] |
58 |
5.4% |
| Total Length |
1075 |
|
| Instability index (II) |
42.66 |
| This classifies the protein as unstable |
|
| Carbon |
5204 |
| Hydrogen |
8245 |
| Nitrogen |
1507 |
| Oxygen |
1676 |
| Sulfur |
24 |
| Total number of atoms |
16656 |
| Formula = C5204H8245N1507O1676S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
91400 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
90650 M-1 cm-1 |
| assuming all Cys residues are reduced |
90650 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
91400 M-1 cm-1 |
|
|
| Codon Bias |
0.022 |
| Codon Adaptation Index |
0.119 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.563 |
| Aromaticity Score |
0.069 |
|
