Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
4.8% |
| Arg[R] |
15 |
3.1% |
| Asn[N] |
29 |
6.1% |
| Asp[D] |
31 |
6.5% |
| Cys[C] |
7 |
1.5% |
| Gln[Q] |
25 |
5.2% |
| Glu[E] |
32 |
6.7% |
| Gly[G] |
16 |
3.3% |
| His[H] |
11 |
2.3% |
| Ile[I] |
40 |
8.4% |
| Leu[L] |
70 |
14.6% |
| Lys[K] |
41 |
8.6% |
| Met[M] |
5 |
1.0% |
| Phe[F] |
17 |
3.6% |
| Pro[P] |
9 |
1.9% |
| Ser[S] |
38 |
7.9% |
| Thr[T] |
21 |
4.4% |
| Trp[W] |
5 |
1.0% |
| Tyr[Y] |
11 |
2.3% |
| Val[V] |
32 |
6.7% |
| Total Length |
478 |
|
| Instability index (II) |
38.48 |
| This classifies the protein as stable |
|
| Carbon |
2444 |
| Hydrogen |
3947 |
| Nitrogen |
645 |
| Oxygen |
729 |
| Sulfur |
12 |
| Total number of atoms |
7777 |
| Formula = C2444H3947N645O729S12 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
44265 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
43890 M-1 cm-1 |
| assuming all Cys residues are reduced |
43890 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
44265 M-1 cm-1 |
|
|
| Codon Bias |
0.252 |
| Codon Adaptation Index |
0.193 |
| Frequency of Optimal Codons |
0.562 |
| Hydropathicity of Protein |
-0.126 |
| Aromaticity Score |
0.069 |
|
