Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
42 |
5.0% |
| Arg[R] |
24 |
2.9% |
| Asn[N] |
58 |
7.0% |
| Asp[D] |
64 |
7.7% |
| Cys[C] |
9 |
1.1% |
| Gln[Q] |
29 |
3.5% |
| Glu[E] |
50 |
6.0% |
| Gly[G] |
33 |
4.0% |
| His[H] |
15 |
1.8% |
| Ile[I] |
76 |
9.1% |
| Leu[L] |
124 |
14.9% |
| Lys[K] |
67 |
8.1% |
| Met[M] |
8 |
1.0% |
| Phe[F] |
32 |
3.8% |
| Pro[P] |
18 |
2.2% |
| Ser[S] |
75 |
9.0% |
| Thr[T] |
44 |
5.3% |
| Trp[W] |
3 |
0.4% |
| Tyr[Y] |
24 |
2.9% |
| Val[V] |
37 |
4.4% |
| Total Length |
832 |
|
| Instability index (II) |
38.69 |
| This classifies the protein as stable |
|
| Carbon |
4191 |
| Hydrogen |
6751 |
| Nitrogen |
1091 |
| Oxygen |
1291 |
| Sulfur |
17 |
| Total number of atoms |
13341 |
| Formula = C4191H6751N1091O1291S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
52760 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
52260 M-1 cm-1 |
| assuming all Cys residues are reduced |
52260 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
52760 M-1 cm-1 |
|
|
| Codon Bias |
0.029 |
| Codon Adaptation Index |
0.149 |
| Frequency of Optimal Codons |
0.429 |
| Hydropathicity of Protein |
-0.139 |
| Aromaticity Score |
0.071 |
|
