Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
26 |
6.6% |
| Arg[R] |
15 |
3.8% |
| Asn[N] |
20 |
5.1% |
| Asp[D] |
21 |
5.3% |
| Cys[C] |
5 |
1.3% |
| Gln[Q] |
16 |
4.1% |
| Glu[E] |
38 |
9.7% |
| Gly[G] |
7 |
1.8% |
| His[H] |
7 |
1.8% |
| Ile[I] |
24 |
6.1% |
| Leu[L] |
38 |
9.7% |
| Lys[K] |
42 |
10.7% |
| Met[M] |
6 |
1.5% |
| Phe[F] |
16 |
4.1% |
| Pro[P] |
16 |
4.1% |
| Ser[S] |
31 |
7.9% |
| Thr[T] |
29 |
7.4% |
| Trp[W] |
1 |
0.3% |
| Tyr[Y] |
17 |
4.3% |
| Val[V] |
18 |
4.6% |
| Total Length |
393 |
|
| Instability index (II) |
40.93 |
| This classifies the protein as unstable |
|
| Carbon |
2014 |
| Hydrogen |
3205 |
| Nitrogen |
531 |
| Oxygen |
625 |
| Sulfur |
11 |
| Total number of atoms |
6386 |
| Formula = C2014H3205N531O625S11 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
31080 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
30830 M-1 cm-1 |
| assuming all Cys residues are reduced |
30830 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
31080 M-1 cm-1 |
|
|
| Codon Bias |
-0.040 |
| Codon Adaptation Index |
0.126 |
| Frequency of Optimal Codons |
0.396 |
| Hydropathicity of Protein |
-0.609 |
| Aromaticity Score |
0.087 |
|
