Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
4.6% |
| Arg[R] |
66 |
7.1% |
| Asn[N] |
52 |
5.6% |
| Asp[D] |
71 |
7.6% |
| Cys[C] |
13 |
1.4% |
| Gln[Q] |
45 |
4.8% |
| Glu[E] |
60 |
6.4% |
| Gly[G] |
42 |
4.5% |
| His[H] |
17 |
1.8% |
| Ile[I] |
64 |
6.9% |
| Leu[L] |
85 |
9.1% |
| Lys[K] |
52 |
5.6% |
| Met[M] |
21 |
2.3% |
| Phe[F] |
21 |
2.3% |
| Pro[P] |
38 |
4.1% |
| Ser[S] |
99 |
10.6% |
| Thr[T] |
49 |
5.3% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
28 |
3.0% |
| Val[V] |
66 |
7.1% |
| Total Length |
933 |
|
| Instability index (II) |
53.56 |
| This classifies the protein as unstable |
|
| Carbon |
4543 |
| Hydrogen |
7367 |
| Nitrogen |
1315 |
| Oxygen |
1469 |
| Sulfur |
34 |
| Total number of atoms |
14728 |
| Formula = C4543H7367N1315O1469S34 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
47970 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
47220 M-1 cm-1 |
| assuming all Cys residues are reduced |
47220 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
47970 M-1 cm-1 |
|
|
| Codon Bias |
0.154 |
| Codon Adaptation Index |
0.177 |
| Frequency of Optimal Codons |
0.496 |
| Hydropathicity of Protein |
-0.519 |
| Aromaticity Score |
0.054 |
|
