Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
50 |
4.2% |
| Arg[R] |
72 |
6.0% |
| Asn[N] |
58 |
4.8% |
| Asp[D] |
71 |
5.9% |
| Cys[C] |
18 |
1.5% |
| Gln[Q] |
59 |
4.9% |
| Glu[E] |
67 |
5.6% |
| Gly[G] |
100 |
8.3% |
| His[H] |
30 |
2.5% |
| Ile[I] |
78 |
6.5% |
| Leu[L] |
103 |
8.6% |
| Lys[K] |
67 |
5.6% |
| Met[M] |
34 |
2.8% |
| Phe[F] |
52 |
4.3% |
| Pro[P] |
61 |
5.1% |
| Ser[S] |
86 |
7.1% |
| Thr[T] |
58 |
4.8% |
| Trp[W] |
10 |
0.8% |
| Tyr[Y] |
49 |
4.1% |
| Val[V] |
80 |
6.7% |
| Total Length |
1203 |
|
| Instability index (II) |
38.80 |
| This classifies the protein as stable |
|
| Carbon |
6034 |
| Hydrogen |
9453 |
| Nitrogen |
1673 |
| Oxygen |
1790 |
| Sulfur |
52 |
| Total number of atoms |
19002 |
| Formula = C6034H9453N1673O1790S52 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
129135 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
128010 M-1 cm-1 |
| assuming all Cys residues are reduced |
128010 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
129135 M-1 cm-1 |
|
|
| Codon Bias |
0.234 |
| Codon Adaptation Index |
0.223 |
| Frequency of Optimal Codons |
0.543 |
| Hydropathicity of Protein |
-0.391 |
| Aromaticity Score |
0.092 |
|
