Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
4.9% |
| Arg[R] |
36 |
4.1% |
| Asn[N] |
62 |
7.0% |
| Asp[D] |
45 |
5.1% |
| Cys[C] |
18 |
2.0% |
| Gln[Q] |
46 |
5.2% |
| Glu[E] |
40 |
4.5% |
| Gly[G] |
29 |
3.3% |
| His[H] |
17 |
1.9% |
| Ile[I] |
56 |
6.4% |
| Leu[L] |
95 |
10.8% |
| Lys[K] |
47 |
5.3% |
| Met[M] |
19 |
2.2% |
| Phe[F] |
38 |
4.3% |
| Pro[P] |
46 |
5.2% |
| Ser[S] |
100 |
11.4% |
| Thr[T] |
66 |
7.5% |
| Trp[W] |
12 |
1.4% |
| Tyr[Y] |
27 |
3.1% |
| Val[V] |
39 |
4.4% |
| Total Length |
881 |
|
| Instability index (II) |
46.47 |
| This classifies the protein as unstable |
|
| Carbon |
4406 |
| Hydrogen |
6926 |
| Nitrogen |
1190 |
| Oxygen |
1353 |
| Sulfur |
37 |
| Total number of atoms |
13912 |
| Formula = C4406H6926N1190O1353S37 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
107355 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
106230 M-1 cm-1 |
| assuming all Cys residues are reduced |
106230 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
107355 M-1 cm-1 |
|
|
| Codon Bias |
0.009 |
| Codon Adaptation Index |
0.116 |
| Frequency of Optimal Codons |
0.411 |
| Hydropathicity of Protein |
-0.330 |
| Aromaticity Score |
0.087 |
|
