Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
6.1% |
| Arg[R] |
27 |
3.8% |
| Asn[N] |
26 |
3.7% |
| Asp[D] |
47 |
6.6% |
| Cys[C] |
0 |
0.0% |
| Gln[Q] |
22 |
3.1% |
| Glu[E] |
95 |
13.4% |
| Gly[G] |
24 |
3.4% |
| His[H] |
4 |
0.6% |
| Ile[I] |
49 |
6.9% |
| Leu[L] |
66 |
9.3% |
| Lys[K] |
77 |
10.9% |
| Met[M] |
13 |
1.8% |
| Phe[F] |
33 |
4.7% |
| Pro[P] |
27 |
3.8% |
| Ser[S] |
49 |
6.9% |
| Thr[T] |
40 |
5.6% |
| Trp[W] |
5 |
0.7% |
| Tyr[Y] |
20 |
2.8% |
| Val[V] |
42 |
5.9% |
| Total Length |
709 |
|
| Instability index (II) |
43.20 |
| This classifies the protein as unstable |
|
| Carbon |
3641 |
| Hydrogen |
5798 |
| Nitrogen |
928 |
| Oxygen |
1151 |
| Sulfur |
13 |
| Total number of atoms |
11531 |
| Formula = C3641H5798N928O1151S13 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
57300 M-1 cm-1 |
|
|
| Codon Bias |
0.600 |
| Codon Adaptation Index |
0.518 |
| Frequency of Optimal Codons |
0.768 |
| Hydropathicity of Protein |
-0.575 |
| Aromaticity Score |
0.082 |
|
