Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
69 |
5.8% |
| Arg[R] |
75 |
6.3% |
| Asn[N] |
51 |
4.3% |
| Asp[D] |
88 |
7.4% |
| Cys[C] |
6 |
0.5% |
| Gln[Q] |
41 |
3.5% |
| Glu[E] |
118 |
10.0% |
| Gly[G] |
75 |
6.3% |
| His[H] |
23 |
1.9% |
| Ile[I] |
63 |
5.3% |
| Leu[L] |
82 |
6.9% |
| Lys[K] |
123 |
10.4% |
| Met[M] |
33 |
2.8% |
| Phe[F] |
53 |
4.5% |
| Pro[P] |
54 |
4.6% |
| Ser[S] |
62 |
5.2% |
| Thr[T] |
66 |
5.6% |
| Trp[W] |
11 |
0.9% |
| Tyr[Y] |
29 |
2.5% |
| Val[V] |
61 |
5.2% |
| Total Length |
1183 |
|
| Instability index (II) |
45.95 |
| This classifies the protein as unstable |
|
| Carbon |
5971 |
| Hydrogen |
9478 |
| Nitrogen |
1680 |
| Oxygen |
1845 |
| Sulfur |
39 |
| Total number of atoms |
19013 |
| Formula = C5971H9478N1680O1845S39 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
104085 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
103710 M-1 cm-1 |
| assuming all Cys residues are reduced |
103710 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
104085 M-1 cm-1 |
|
|
| Codon Bias |
0.152 |
| Codon Adaptation Index |
0.193 |
| Frequency of Optimal Codons |
0.500 |
| Hydropathicity of Protein |
-0.839 |
| Aromaticity Score |
0.079 |
|
