Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
4.4% |
| Arg[R] |
29 |
4.7% |
| Asn[N] |
47 |
7.7% |
| Asp[D] |
41 |
6.7% |
| Cys[C] |
12 |
2.0% |
| Gln[Q] |
24 |
3.9% |
| Glu[E] |
46 |
7.5% |
| Gly[G] |
25 |
4.1% |
| His[H] |
13 |
2.1% |
| Ile[I] |
41 |
6.7% |
| Leu[L] |
48 |
7.8% |
| Lys[K] |
52 |
8.5% |
| Met[M] |
12 |
2.0% |
| Phe[F] |
27 |
4.4% |
| Pro[P] |
21 |
3.4% |
| Ser[S] |
48 |
7.8% |
| Thr[T] |
50 |
8.2% |
| Trp[W] |
5 |
0.8% |
| Tyr[Y] |
13 |
2.1% |
| Val[V] |
31 |
5.1% |
| Total Length |
612 |
|
| Instability index (II) |
38.38 |
| This classifies the protein as stable |
|
| Carbon |
3046 |
| Hydrogen |
4849 |
| Nitrogen |
853 |
| Oxygen |
969 |
| Sulfur |
24 |
| Total number of atoms |
9741 |
| Formula = C3046H4849N853O969S24 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
47620 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
46870 M-1 cm-1 |
| assuming all Cys residues are reduced |
46870 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
47620 M-1 cm-1 |
|
|
| Codon Bias |
-0.022 |
| Codon Adaptation Index |
0.123 |
| Frequency of Optimal Codons |
0.412 |
| Hydropathicity of Protein |
-0.641 |
| Aromaticity Score |
0.074 |
|
