Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
41 |
5.1% |
| Arg[R] |
29 |
3.6% |
| Asn[N] |
63 |
7.9% |
| Asp[D] |
66 |
8.2% |
| Cys[C] |
2 |
0.2% |
| Gln[Q] |
83 |
10.3% |
| Glu[E] |
82 |
10.2% |
| Gly[G] |
44 |
5.5% |
| His[H] |
18 |
2.2% |
| Ile[I] |
35 |
4.4% |
| Leu[L] |
40 |
5.0% |
| Lys[K] |
37 |
4.6% |
| Met[M] |
24 |
3.0% |
| Phe[F] |
21 |
2.6% |
| Pro[P] |
66 |
8.2% |
| Ser[S] |
67 |
8.4% |
| Thr[T] |
29 |
3.6% |
| Trp[W] |
1 |
0.1% |
| Tyr[Y] |
25 |
3.1% |
| Val[V] |
29 |
3.6% |
| Total Length |
802 |
|
| Instability index (II) |
73.41 |
| This classifies the protein as unstable |
|
| Carbon |
3849 |
| Hydrogen |
5939 |
| Nitrogen |
1109 |
| Oxygen |
1366 |
| Sulfur |
26 |
| Total number of atoms |
12289 |
| Formula = C3849H5939N1109O1366S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
42875 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
42750 M-1 cm-1 |
| assuming all Cys residues are reduced |
42750 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
42875 M-1 cm-1 |
|
|
| Codon Bias |
0.155 |
| Codon Adaptation Index |
0.205 |
| Frequency of Optimal Codons |
0.506 |
| Hydropathicity of Protein |
-1.219 |
| Aromaticity Score |
0.059 |
|
