Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
5.4% |
| Arg[R] |
45 |
5.6% |
| Asn[N] |
74 |
9.3% |
| Asp[D] |
55 |
6.9% |
| Cys[C] |
2 |
0.3% |
| Gln[Q] |
40 |
5.0% |
| Glu[E] |
47 |
5.9% |
| Gly[G] |
40 |
5.0% |
| His[H] |
34 |
4.3% |
| Ile[I] |
36 |
4.5% |
| Leu[L] |
59 |
7.4% |
| Lys[K] |
58 |
7.3% |
| Met[M] |
12 |
1.5% |
| Phe[F] |
26 |
3.3% |
| Pro[P] |
27 |
3.4% |
| Ser[S] |
117 |
14.6% |
| Thr[T] |
44 |
5.5% |
| Trp[W] |
2 |
0.3% |
| Tyr[Y] |
10 |
1.3% |
| Val[V] |
28 |
3.5% |
| Total Length |
799 |
|
| Instability index (II) |
59.50 |
| This classifies the protein as unstable |
|
| Carbon |
3766 |
| Hydrogen |
6020 |
| Nitrogen |
1176 |
| Oxygen |
1289 |
| Sulfur |
14 |
| Total number of atoms |
12265 |
| Formula = C3766H6020N1176O1289S14 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
26025 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
25900 M-1 cm-1 |
| assuming all Cys residues are reduced |
25900 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
26025 M-1 cm-1 |
|
|
| Codon Bias |
0.005 |
| Codon Adaptation Index |
0.125 |
| Frequency of Optimal Codons |
0.406 |
| Hydropathicity of Protein |
-1.014 |
| Aromaticity Score |
0.048 |
|
