Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
35 |
5.8% |
| Arg[R] |
22 |
3.7% |
| Asn[N] |
53 |
8.8% |
| Asp[D] |
21 |
3.5% |
| Cys[C] |
1 |
0.2% |
| Gln[Q] |
42 |
7.0% |
| Glu[E] |
28 |
4.7% |
| Gly[G] |
26 |
4.3% |
| His[H] |
8 |
1.3% |
| Ile[I] |
34 |
5.7% |
| Leu[L] |
58 |
9.7% |
| Lys[K] |
36 |
6.0% |
| Met[M] |
21 |
3.5% |
| Phe[F] |
28 |
4.7% |
| Pro[P] |
33 |
5.5% |
| Ser[S] |
59 |
9.8% |
| Thr[T] |
35 |
5.8% |
| Trp[W] |
7 |
1.2% |
| Tyr[Y] |
16 |
2.7% |
| Val[V] |
36 |
6.0% |
| Total Length |
599 |
|
| Instability index (II) |
39.79 |
| This classifies the protein as stable |
|
| Carbon |
2994 |
| Hydrogen |
4729 |
| Nitrogen |
819 |
| Oxygen |
903 |
| Sulfur |
22 |
| Total number of atoms |
9467 |
| Formula = C2994H4729N819O903S22 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
62340 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
62340 M-1 cm-1 |
| assuming all Cys residues are reduced |
62340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
62340 M-1 cm-1 |
|
|
| Codon Bias |
0.050 |
| Codon Adaptation Index |
0.120 |
| Frequency of Optimal Codons |
0.434 |
| Hydropathicity of Protein |
-0.372 |
| Aromaticity Score |
0.085 |
|
