Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
4.5% |
| Arg[R] |
35 |
4.3% |
| Asn[N] |
51 |
6.2% |
| Asp[D] |
49 |
6.0% |
| Cys[C] |
10 |
1.2% |
| Gln[Q] |
57 |
6.9% |
| Glu[E] |
50 |
6.1% |
| Gly[G] |
56 |
6.8% |
| His[H] |
15 |
1.8% |
| Ile[I] |
62 |
7.6% |
| Leu[L] |
68 |
8.3% |
| Lys[K] |
66 |
8.0% |
| Met[M] |
19 |
2.3% |
| Phe[F] |
32 |
3.9% |
| Pro[P] |
27 |
3.3% |
| Ser[S] |
73 |
8.9% |
| Thr[T] |
42 |
5.1% |
| Trp[W] |
6 |
0.7% |
| Tyr[Y] |
17 |
2.1% |
| Val[V] |
49 |
6.0% |
| Total Length |
821 |
|
| Instability index (II) |
41.07 |
| This classifies the protein as unstable |
|
| Carbon |
4033 |
| Hydrogen |
6470 |
| Nitrogen |
1136 |
| Oxygen |
1260 |
| Sulfur |
29 |
| Total number of atoms |
12928 |
| Formula = C4033H6470N1136O1260S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58955 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
58330 M-1 cm-1 |
| assuming all Cys residues are reduced |
58330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58955 M-1 cm-1 |
|
|
| Codon Bias |
0.011 |
| Codon Adaptation Index |
0.121 |
| Frequency of Optimal Codons |
0.423 |
| Hydropathicity of Protein |
-0.497 |
| Aromaticity Score |
0.067 |
|
