RAD53/YPL153C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 37 4.5%
Arg[R] 35 4.3%
Asn[N] 51 6.2%
Asp[D] 49 6.0%
Cys[C] 10 1.2%
Gln[Q] 57 6.9%
Glu[E] 50 6.1%
Gly[G] 56 6.8%
His[H] 15 1.8%
Ile[I] 62 7.6%
Leu[L] 68 8.3%
Lys[K] 66 8.0%
Met[M] 19 2.3%
Phe[F] 32 3.9%
Pro[P] 27 3.3%
Ser[S] 73 8.9%
Thr[T] 42 5.1%
Trp[W] 6 0.7%
Tyr[Y] 17 2.1%
Val[V] 49 6.0%
Total Length 821

Instability index
Instability index (II) 41.07
This classifies the protein as unstable

Atomic composition
Carbon 4033
Hydrogen 6470
Nitrogen 1136
Oxygen 1260
Sulfur 29
Total number of atoms 12928
Formula = C4033H6470N1136O1260S29

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 58955 M-1 cm-1
assuming NO Cys residues appear as half cystines 58330 M-1 cm-1
assuming all Cys residues are reduced 58330 M-1 cm-1
assuming all pairs of Cys residues form cystines 58955 M-1 cm-1

Aliphatic index
Aliphatic index 83.57

Coding Region Translation Calculations
Codon Bias 0.011
Codon Adaptation Index 0.121
Frequency of Optimal Codons 0.423
Hydropathicity of Protein -0.497
Aromaticity Score 0.067