Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
4.9% |
| Arg[R] |
54 |
6.2% |
| Asn[N] |
54 |
6.2% |
| Asp[D] |
49 |
5.6% |
| Cys[C] |
6 |
0.7% |
| Gln[Q] |
25 |
2.9% |
| Glu[E] |
39 |
4.5% |
| Gly[G] |
43 |
4.9% |
| His[H] |
14 |
1.6% |
| Ile[I] |
77 |
8.9% |
| Leu[L] |
107 |
12.3% |
| Lys[K] |
65 |
7.5% |
| Met[M] |
19 |
2.2% |
| Phe[F] |
49 |
5.6% |
| Pro[P] |
29 |
3.3% |
| Ser[S] |
63 |
7.2% |
| Thr[T] |
41 |
4.7% |
| Trp[W] |
9 |
1.0% |
| Tyr[Y] |
34 |
3.9% |
| Val[V] |
50 |
5.7% |
| Total Length |
870 |
|
| Instability index (II) |
34.80 |
| This classifies the protein as stable |
|
| Carbon |
4556 |
| Hydrogen |
7231 |
| Nitrogen |
1213 |
| Oxygen |
1264 |
| Sulfur |
25 |
| Total number of atoms |
14289 |
| Formula = C4556H7231N1213O1264S25 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
100535 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
100160 M-1 cm-1 |
| assuming all Cys residues are reduced |
100160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
100535 M-1 cm-1 |
|
|
| Codon Bias |
0.044 |
| Codon Adaptation Index |
0.131 |
| Frequency of Optimal Codons |
0.431 |
| Hydropathicity of Protein |
-0.106 |
| Aromaticity Score |
0.106 |
|
