Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
32 |
10.7% |
| Arg[R] |
22 |
7.3% |
| Asn[N] |
10 |
3.3% |
| Asp[D] |
20 |
6.7% |
| Cys[C] |
5 |
1.7% |
| Gln[Q] |
9 |
3.0% |
| Glu[E] |
17 |
5.7% |
| Gly[G] |
11 |
3.7% |
| His[H] |
4 |
1.3% |
| Ile[I] |
24 |
8.0% |
| Leu[L] |
22 |
7.3% |
| Lys[K] |
20 |
6.7% |
| Met[M] |
5 |
1.7% |
| Phe[F] |
20 |
6.7% |
| Pro[P] |
16 |
5.3% |
| Ser[S] |
20 |
6.7% |
| Thr[T] |
14 |
4.7% |
| Trp[W] |
2 |
0.7% |
| Tyr[Y] |
11 |
3.7% |
| Val[V] |
16 |
5.3% |
| Total Length |
300 |
|
| Instability index (II) |
49.61 |
| This classifies the protein as unstable |
|
| Carbon |
1537 |
| Hydrogen |
2407 |
| Nitrogen |
415 |
| Oxygen |
439 |
| Sulfur |
10 |
| Total number of atoms |
4808 |
| Formula = C1537H2407N415O439S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
27640 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
27390 M-1 cm-1 |
| assuming all Cys residues are reduced |
27390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
27640 M-1 cm-1 |
|
|
| Codon Bias |
0.070 |
| Codon Adaptation Index |
0.151 |
| Frequency of Optimal Codons |
0.444 |
| Hydropathicity of Protein |
-0.211 |
| Aromaticity Score |
0.110 |
|
