Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
27 |
3.0% |
| Arg[R] |
21 |
2.3% |
| Asn[N] |
85 |
9.5% |
| Asp[D] |
58 |
6.5% |
| Cys[C] |
6 |
0.7% |
| Gln[Q] |
45 |
5.0% |
| Glu[E] |
38 |
4.2% |
| Gly[G] |
43 |
4.8% |
| His[H] |
24 |
2.7% |
| Ile[I] |
70 |
7.8% |
| Leu[L] |
95 |
10.6% |
| Lys[K] |
60 |
6.7% |
| Met[M] |
14 |
1.6% |
| Phe[F] |
45 |
5.0% |
| Pro[P] |
23 |
2.6% |
| Ser[S] |
94 |
10.5% |
| Thr[T] |
59 |
6.6% |
| Trp[W] |
12 |
1.3% |
| Tyr[Y] |
24 |
2.7% |
| Val[V] |
53 |
5.9% |
| Total Length |
896 |
|
| Instability index (II) |
33.61 |
| This classifies the protein as stable |
|
| Carbon |
4513 |
| Hydrogen |
7065 |
| Nitrogen |
1209 |
| Oxygen |
1396 |
| Sulfur |
20 |
| Total number of atoms |
14203 |
| Formula = C4513H7065N1209O1396S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
102135 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
101760 M-1 cm-1 |
| assuming all Cys residues are reduced |
101760 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
102135 M-1 cm-1 |
|
|
| Codon Bias |
0.122 |
| Codon Adaptation Index |
0.169 |
| Frequency of Optimal Codons |
0.494 |
| Hydropathicity of Protein |
-0.328 |
| Aromaticity Score |
0.090 |
|
