TFB2/YPL122C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 25 4.9%
Arg[R] 17 3.3%
Asn[N] 31 6.0%
Asp[D] 28 5.5%
Cys[C] 2 0.4%
Gln[Q] 27 5.3%
Glu[E] 34 6.6%
Gly[G] 20 3.9%
His[H] 9 1.8%
Ile[I] 32 6.2%
Leu[L] 70 13.6%
Lys[K] 42 8.2%
Met[M] 16 3.1%
Phe[F] 21 4.1%
Pro[P] 18 3.5%
Ser[S] 40 7.8%
Thr[T] 29 5.7%
Trp[W] 5 1.0%
Tyr[Y] 16 3.1%
Val[V] 31 6.0%
Total Length 513

Instability index
Instability index (II) 29.63
This classifies the protein as stable

Atomic composition
Carbon 2631
Hydrogen 4205
Nitrogen 687
Oxygen 781
Sulfur 18
Total number of atoms 8322
Formula = C2631H4205N687O781S18

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 51465 M-1 cm-1
assuming NO Cys residues appear as half cystines 51340 M-1 cm-1
assuming all Cys residues are reduced 51340 M-1 cm-1
assuming all pairs of Cys residues form cystines 51465 M-1 cm-1

Aliphatic index
Aliphatic index 99.94

Coding Region Translation Calculations
Codon Bias 0.110
Codon Adaptation Index 0.172
Frequency of Optimal Codons 0.472
Hydropathicity of Protein -0.242
Aromaticity Score 0.082