Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
3 |
0.6% |
| Arg[R] |
19 |
4.0% |
| Asn[N] |
50 |
10.4% |
| Asp[D] |
22 |
4.6% |
| Cys[C] |
6 |
1.3% |
| Gln[Q] |
19 |
4.0% |
| Glu[E] |
17 |
3.5% |
| Gly[G] |
12 |
2.5% |
| His[H] |
9 |
1.9% |
| Ile[I] |
46 |
9.6% |
| Leu[L] |
42 |
8.8% |
| Lys[K] |
37 |
7.7% |
| Met[M] |
4 |
0.8% |
| Phe[F] |
29 |
6.1% |
| Pro[P] |
34 |
7.1% |
| Ser[S] |
58 |
12.1% |
| Thr[T] |
34 |
7.1% |
| Trp[W] |
1 |
0.2% |
| Tyr[Y] |
18 |
3.8% |
| Val[V] |
19 |
4.0% |
| Total Length |
479 |
|
| Instability index (II) |
51.46 |
| This classifies the protein as unstable |
|
| Carbon |
2466 |
| Hydrogen |
3873 |
| Nitrogen |
661 |
| Oxygen |
737 |
| Sulfur |
10 |
| Total number of atoms |
7747 |
| Formula = C2466H3873N661O737S10 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
32695 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
32320 M-1 cm-1 |
| assuming all Cys residues are reduced |
32320 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
32695 M-1 cm-1 |
|
|
| Codon Bias |
0.039 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.449 |
| Hydropathicity of Protein |
-0.490 |
| Aromaticity Score |
0.100 |
|
