LCL1/YPL056C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 7 6.9%
Arg[R] 7 6.9%
Asn[N] 3 3.0%
Asp[D] 2 2.0%
Cys[C] 5 5.0%
Gln[Q] 3 3.0%
Glu[E] 2 2.0%
Gly[G] 0 0.0%
His[H] 2 2.0%
Ile[I] 4 4.0%
Leu[L] 18 17.8%
Lys[K] 4 4.0%
Met[M] 1 1.0%
Phe[F] 10 9.9%
Pro[P] 12 11.9%
Ser[S] 12 11.9%
Thr[T] 5 5.0%
Trp[W] 0 0.0%
Tyr[Y] 0 0.0%
Val[V] 4 4.0%
Total Length 101

Instability index
Instability index (II) 57.82
This classifies the protein as unstable

Atomic composition
Carbon 522
Hydrogen 830
Nitrogen 136
Oxygen 133
Sulfur 6
Total number of atoms 1627
Formula = C522H830N136O133S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 250 M-1 cm-1
assuming NO Cys residues appear as half cystines 0 M-1 cm-1
assuming all Cys residues are reduced 0 M-1 cm-1
assuming all pairs of Cys residues form cystines 250 M-1 cm-1

Aliphatic index
Aliphatic index 103.37

Coding Region Translation Calculations
Codon Bias 0.131
Codon Adaptation Index 0.100
Frequency of Optimal Codons 0.440
Hydropathicity of Protein 0.370
Aromaticity Score 0.099