Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
23 |
5.2% |
| Arg[R] |
17 |
3.8% |
| Asn[N] |
28 |
6.3% |
| Asp[D] |
31 |
7.0% |
| Cys[C] |
7 |
1.6% |
| Gln[Q] |
17 |
3.8% |
| Glu[E] |
34 |
7.6% |
| Gly[G] |
23 |
5.2% |
| His[H] |
9 |
2.0% |
| Ile[I] |
36 |
8.1% |
| Leu[L] |
27 |
6.1% |
| Lys[K] |
35 |
7.8% |
| Met[M] |
12 |
2.7% |
| Phe[F] |
34 |
7.6% |
| Pro[P] |
20 |
4.5% |
| Ser[S] |
30 |
6.7% |
| Thr[T] |
15 |
3.4% |
| Trp[W] |
12 |
2.7% |
| Tyr[Y] |
18 |
4.0% |
| Val[V] |
18 |
4.0% |
| Total Length |
446 |
|
| Instability index (II) |
45.96 |
| This classifies the protein as unstable |
|
| Carbon |
2371 |
| Hydrogen |
3551 |
| Nitrogen |
607 |
| Oxygen |
685 |
| Sulfur |
19 |
| Total number of atoms |
7233 |
| Formula = C2371H3551N607O685S19 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
93195 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
92820 M-1 cm-1 |
| assuming all Cys residues are reduced |
92820 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
93195 M-1 cm-1 |
|
|
| Codon Bias |
0.046 |
| Codon Adaptation Index |
0.160 |
| Frequency of Optimal Codons |
0.455 |
| Hydropathicity of Protein |
-0.492 |
| Aromaticity Score |
0.143 |
|
