ARL3/YPL051W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 4.5%
Arg[R] 10 5.1%
Asn[N] 8 4.0%
Asp[D] 12 6.1%
Cys[C] 2 1.0%
Gln[Q] 9 4.5%
Glu[E] 17 8.6%
Gly[G] 10 5.1%
His[H] 3 1.5%
Ile[I] 17 8.6%
Leu[L] 19 9.6%
Lys[K] 17 8.6%
Met[M] 6 3.0%
Phe[F] 6 3.0%
Pro[P] 6 3.0%
Ser[S] 15 7.6%
Thr[T] 8 4.0%
Trp[W] 4 2.0%
Tyr[Y] 6 3.0%
Val[V] 14 7.1%
Total Length 198

Instability index
Instability index (II) 52.60
This classifies the protein as unstable

Atomic composition
Carbon 1018
Hydrogen 1628
Nitrogen 272
Oxygen 303
Sulfur 8
Total number of atoms 3229
Formula = C1018H1628N272O303S8

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 31065 M-1 cm-1
assuming NO Cys residues appear as half cystines 30940 M-1 cm-1
assuming all Cys residues are reduced 30940 M-1 cm-1
assuming all pairs of Cys residues form cystines 31065 M-1 cm-1

Aliphatic index
Aliphatic index 95.96

Coding Region Translation Calculations
Codon Bias 0.060
Codon Adaptation Index 0.128
Frequency of Optimal Codons 0.452
Hydropathicity of Protein -0.341
Aromaticity Score 0.081