Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
38 |
4.8% |
| Arg[R] |
30 |
3.8% |
| Asn[N] |
38 |
4.8% |
| Asp[D] |
53 |
6.6% |
| Cys[C] |
15 |
1.9% |
| Gln[Q] |
37 |
4.6% |
| Glu[E] |
53 |
6.6% |
| Gly[G] |
30 |
3.8% |
| His[H] |
12 |
1.5% |
| Ile[I] |
66 |
8.3% |
| Leu[L] |
98 |
12.3% |
| Lys[K] |
74 |
9.3% |
| Met[M] |
17 |
2.1% |
| Phe[F] |
36 |
4.5% |
| Pro[P] |
24 |
3.0% |
| Ser[S] |
52 |
6.5% |
| Thr[T] |
36 |
4.5% |
| Trp[W] |
11 |
1.4% |
| Tyr[Y] |
40 |
5.0% |
| Val[V] |
38 |
4.8% |
| Total Length |
798 |
|
| Instability index (II) |
36.65 |
| This classifies the protein as stable |
|
| Carbon |
4213 |
| Hydrogen |
6622 |
| Nitrogen |
1072 |
| Oxygen |
1214 |
| Sulfur |
32 |
| Total number of atoms |
13153 |
| Formula = C4213H6622N1072O1214S32 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
120975 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
120100 M-1 cm-1 |
| assuming all Cys residues are reduced |
120100 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
120975 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.418 |
| Hydropathicity of Protein |
-0.259 |
| Aromaticity Score |
0.109 |
|
