Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
103 |
10.9% |
| Arg[R] |
34 |
3.6% |
| Asn[N] |
31 |
3.3% |
| Asp[D] |
53 |
5.6% |
| Cys[C] |
9 |
1.0% |
| Gln[Q] |
26 |
2.7% |
| Glu[E] |
61 |
6.4% |
| Gly[G] |
76 |
8.0% |
| His[H] |
13 |
1.4% |
| Ile[I] |
69 |
7.3% |
| Leu[L] |
89 |
9.4% |
| Lys[K] |
46 |
4.9% |
| Met[M] |
22 |
2.3% |
| Phe[F] |
37 |
3.9% |
| Pro[P] |
34 |
3.6% |
| Ser[S] |
71 |
7.5% |
| Thr[T] |
56 |
5.9% |
| Trp[W] |
15 |
1.6% |
| Tyr[Y] |
23 |
2.4% |
| Val[V] |
79 |
8.3% |
| Total Length |
947 |
|
| Instability index (II) |
34.66 |
| This classifies the protein as stable |
|
| Carbon |
4582 |
| Hydrogen |
7249 |
| Nitrogen |
1193 |
| Oxygen |
1383 |
| Sulfur |
31 |
| Total number of atoms |
14438 |
| Formula = C4582H7249N1193O1383S31 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
117270 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
116770 M-1 cm-1 |
| assuming all Cys residues are reduced |
116770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
117270 M-1 cm-1 |
|
|
| Codon Bias |
0.385 |
| Codon Adaptation Index |
0.300 |
| Frequency of Optimal Codons |
0.629 |
| Hydropathicity of Protein |
0.145 |
| Aromaticity Score |
0.079 |
|
