Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
46 |
4.2% |
| Arg[R] |
53 |
4.8% |
| Asn[N] |
76 |
6.9% |
| Asp[D] |
78 |
7.1% |
| Cys[C] |
10 |
0.9% |
| Gln[Q] |
45 |
4.1% |
| Glu[E] |
96 |
8.7% |
| Gly[G] |
38 |
3.5% |
| His[H] |
24 |
2.2% |
| Ile[I] |
75 |
6.8% |
| Leu[L] |
126 |
11.5% |
| Lys[K] |
85 |
7.7% |
| Met[M] |
18 |
1.6% |
| Phe[F] |
40 |
3.6% |
| Pro[P] |
40 |
3.6% |
| Ser[S] |
88 |
8.0% |
| Thr[T] |
54 |
4.9% |
| Trp[W] |
12 |
1.1% |
| Tyr[Y] |
29 |
2.6% |
| Val[V] |
67 |
6.1% |
| Total Length |
1100 |
|
| Instability index (II) |
48.38 |
| This classifies the protein as unstable |
|
| Carbon |
5601 |
| Hydrogen |
8913 |
| Nitrogen |
1525 |
| Oxygen |
1741 |
| Sulfur |
28 |
| Total number of atoms |
17808 |
| Formula = C5601H8913N1525O1741S28 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
109835 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
109210 M-1 cm-1 |
| assuming all Cys residues are reduced |
109210 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
109835 M-1 cm-1 |
|
|
| Codon Bias |
-0.008 |
| Codon Adaptation Index |
0.124 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.512 |
| Aromaticity Score |
0.074 |
|
