Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
37 |
4.4% |
| Arg[R] |
44 |
5.3% |
| Asn[N] |
61 |
7.3% |
| Asp[D] |
59 |
7.1% |
| Cys[C] |
2 |
0.2% |
| Gln[Q] |
29 |
3.5% |
| Glu[E] |
59 |
7.1% |
| Gly[G] |
28 |
3.4% |
| His[H] |
14 |
1.7% |
| Ile[I] |
48 |
5.7% |
| Leu[L] |
90 |
10.8% |
| Lys[K] |
73 |
8.7% |
| Met[M] |
7 |
0.8% |
| Phe[F] |
44 |
5.3% |
| Pro[P] |
40 |
4.8% |
| Ser[S] |
70 |
8.4% |
| Thr[T] |
36 |
4.3% |
| Trp[W] |
10 |
1.2% |
| Tyr[Y] |
32 |
3.8% |
| Val[V] |
52 |
6.2% |
| Total Length |
835 |
|
| Instability index (II) |
37.33 |
| This classifies the protein as stable |
|
| Carbon |
4350 |
| Hydrogen |
6804 |
| Nitrogen |
1168 |
| Oxygen |
1300 |
| Sulfur |
9 |
| Total number of atoms |
13631 |
| Formula = C4350H6804N1168O1300S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
102805 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
102680 M-1 cm-1 |
| assuming all Cys residues are reduced |
102680 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
102805 M-1 cm-1 |
|
|
| Codon Bias |
0.111 |
| Codon Adaptation Index |
0.188 |
| Frequency of Optimal Codons |
0.474 |
| Hydropathicity of Protein |
-0.572 |
| Aromaticity Score |
0.103 |
|
