Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
10 |
2.7% |
| Arg[R] |
23 |
6.2% |
| Asn[N] |
30 |
8.1% |
| Asp[D] |
25 |
6.8% |
| Cys[C] |
7 |
1.9% |
| Gln[Q] |
18 |
4.9% |
| Glu[E] |
21 |
5.7% |
| Gly[G] |
15 |
4.1% |
| His[H] |
7 |
1.9% |
| Ile[I] |
28 |
7.6% |
| Leu[L] |
48 |
13.0% |
| Lys[K] |
29 |
7.9% |
| Met[M] |
9 |
2.4% |
| Phe[F] |
14 |
3.8% |
| Pro[P] |
8 |
2.2% |
| Ser[S] |
24 |
6.5% |
| Thr[T] |
24 |
6.5% |
| Trp[W] |
2 |
0.5% |
| Tyr[Y] |
11 |
3.0% |
| Val[V] |
16 |
4.3% |
| Total Length |
369 |
|
| Instability index (II) |
34.69 |
| This classifies the protein as stable |
|
| Carbon |
1886 |
| Hydrogen |
3051 |
| Nitrogen |
531 |
| Oxygen |
569 |
| Sulfur |
16 |
| Total number of atoms |
6053 |
| Formula = C1886H3051N531O569S16 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
27765 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
27390 M-1 cm-1 |
| assuming all Cys residues are reduced |
27390 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
27765 M-1 cm-1 |
|
|
| Codon Bias |
-0.008 |
| Codon Adaptation Index |
0.108 |
| Frequency of Optimal Codons |
0.408 |
| Hydropathicity of Protein |
-0.465 |
| Aromaticity Score |
0.073 |
|
