Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
30 |
5.0% |
| Arg[R] |
42 |
6.9% |
| Asn[N] |
40 |
6.6% |
| Asp[D] |
26 |
4.3% |
| Cys[C] |
10 |
1.7% |
| Gln[Q] |
20 |
3.3% |
| Glu[E] |
38 |
6.3% |
| Gly[G] |
24 |
4.0% |
| His[H] |
12 |
2.0% |
| Ile[I] |
33 |
5.4% |
| Leu[L] |
62 |
10.2% |
| Lys[K] |
61 |
10.1% |
| Met[M] |
11 |
1.8% |
| Phe[F] |
38 |
6.3% |
| Pro[P] |
20 |
3.3% |
| Ser[S] |
47 |
7.8% |
| Thr[T] |
38 |
6.3% |
| Trp[W] |
4 |
0.7% |
| Tyr[Y] |
21 |
3.5% |
| Val[V] |
29 |
4.8% |
| Total Length |
606 |
|
| Instability index (II) |
32.63 |
| This classifies the protein as stable |
|
| Carbon |
3150 |
| Hydrogen |
5007 |
| Nitrogen |
881 |
| Oxygen |
901 |
| Sulfur |
21 |
| Total number of atoms |
9960 |
| Formula = C3150H5007N881O901S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
53915 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
53290 M-1 cm-1 |
| assuming all Cys residues are reduced |
53290 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
53915 M-1 cm-1 |
|
|
| Codon Bias |
-0.043 |
| Codon Adaptation Index |
0.105 |
| Frequency of Optimal Codons |
0.381 |
| Hydropathicity of Protein |
-0.534 |
| Aromaticity Score |
0.104 |
|
