Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
4.4% |
| Arg[R] |
29 |
5.1% |
| Asn[N] |
36 |
6.4% |
| Asp[D] |
29 |
5.1% |
| Cys[C] |
9 |
1.6% |
| Gln[Q] |
21 |
3.7% |
| Glu[E] |
31 |
5.5% |
| Gly[G] |
24 |
4.2% |
| His[H] |
13 |
2.3% |
| Ile[I] |
34 |
6.0% |
| Leu[L] |
51 |
9.0% |
| Lys[K] |
49 |
8.7% |
| Met[M] |
18 |
3.2% |
| Phe[F] |
25 |
4.4% |
| Pro[P] |
22 |
3.9% |
| Ser[S] |
46 |
8.1% |
| Thr[T] |
30 |
5.3% |
| Trp[W] |
16 |
2.8% |
| Tyr[Y] |
31 |
5.5% |
| Val[V] |
26 |
4.6% |
| Total Length |
565 |
|
| Instability index (II) |
39.18 |
| This classifies the protein as stable |
|
| Carbon |
2994 |
| Hydrogen |
4606 |
| Nitrogen |
800 |
| Oxygen |
850 |
| Sulfur |
27 |
| Total number of atoms |
9277 |
| Formula = C2994H4606N800O850S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
134690 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
134190 M-1 cm-1 |
| assuming all Cys residues are reduced |
134190 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
134690 M-1 cm-1 |
|
|
| Codon Bias |
0.051 |
| Codon Adaptation Index |
0.147 |
| Frequency of Optimal Codons |
0.443 |
| Hydropathicity of Protein |
-0.535 |
| Aromaticity Score |
0.127 |
|
