Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
45 |
6.5% |
| Arg[R] |
34 |
4.9% |
| Asn[N] |
22 |
3.2% |
| Asp[D] |
28 |
4.0% |
| Cys[C] |
12 |
1.7% |
| Gln[Q] |
12 |
1.7% |
| Glu[E] |
43 |
6.2% |
| Gly[G] |
44 |
6.3% |
| His[H] |
24 |
3.5% |
| Ile[I] |
50 |
7.2% |
| Leu[L] |
75 |
10.8% |
| Lys[K] |
48 |
6.9% |
| Met[M] |
15 |
2.2% |
| Phe[F] |
44 |
6.3% |
| Pro[P] |
18 |
2.6% |
| Ser[S] |
42 |
6.1% |
| Thr[T] |
36 |
5.2% |
| Trp[W] |
21 |
3.0% |
| Tyr[Y] |
34 |
4.9% |
| Val[V] |
47 |
6.8% |
| Total Length |
694 |
|
| Instability index (II) |
37.32 |
| This classifies the protein as stable |
|
| Carbon |
3723 |
| Hydrogen |
5673 |
| Nitrogen |
947 |
| Oxygen |
983 |
| Sulfur |
27 |
| Total number of atoms |
11353 |
| Formula = C3723H5673N947O983S27 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
166910 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
166160 M-1 cm-1 |
| assuming all Cys residues are reduced |
166160 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
166910 M-1 cm-1 |
|
|
| Codon Bias |
-0.042 |
| Codon Adaptation Index |
0.091 |
| Frequency of Optimal Codons |
0.381 |
| Hydropathicity of Protein |
0.025 |
| Aromaticity Score |
0.143 |
|
