Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
43 |
7.9% |
| Arg[R] |
32 |
5.9% |
| Asn[N] |
25 |
4.6% |
| Asp[D] |
27 |
4.9% |
| Cys[C] |
13 |
2.4% |
| Gln[Q] |
25 |
4.6% |
| Glu[E] |
27 |
4.9% |
| Gly[G] |
21 |
3.8% |
| His[H] |
22 |
4.0% |
| Ile[I] |
34 |
6.2% |
| Leu[L] |
56 |
10.3% |
| Lys[K] |
24 |
4.4% |
| Met[M] |
7 |
1.3% |
| Phe[F] |
14 |
2.6% |
| Pro[P] |
31 |
5.7% |
| Ser[S] |
41 |
7.5% |
| Thr[T] |
36 |
6.6% |
| Trp[W] |
9 |
1.6% |
| Tyr[Y] |
16 |
2.9% |
| Val[V] |
43 |
7.9% |
| Total Length |
546 |
|
| Instability index (II) |
42.44 |
| This classifies the protein as unstable |
|
| Carbon |
2727 |
| Hydrogen |
4319 |
| Nitrogen |
769 |
| Oxygen |
798 |
| Sulfur |
20 |
| Total number of atoms |
8633 |
| Formula = C2727H4319N769O798S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
74090 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
73340 M-1 cm-1 |
| assuming all Cys residues are reduced |
73340 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
74090 M-1 cm-1 |
|
|
| Codon Bias |
0.035 |
| Codon Adaptation Index |
0.108 |
| Frequency of Optimal Codons |
0.419 |
| Hydropathicity of Protein |
-0.198 |
| Aromaticity Score |
0.071 |
|
