Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
25 |
4.8% |
| Arg[R] |
20 |
3.8% |
| Asn[N] |
37 |
7.0% |
| Asp[D] |
32 |
6.1% |
| Cys[C] |
11 |
2.1% |
| Gln[Q] |
25 |
4.8% |
| Glu[E] |
31 |
5.9% |
| Gly[G] |
13 |
2.5% |
| His[H] |
25 |
4.8% |
| Ile[I] |
42 |
8.0% |
| Leu[L] |
61 |
11.6% |
| Lys[K] |
32 |
6.1% |
| Met[M] |
10 |
1.9% |
| Phe[F] |
31 |
5.9% |
| Pro[P] |
18 |
3.4% |
| Ser[S] |
41 |
7.8% |
| Thr[T] |
25 |
4.8% |
| Trp[W] |
8 |
1.5% |
| Tyr[Y] |
13 |
2.5% |
| Val[V] |
26 |
4.9% |
| Total Length |
526 |
|
| Instability index (II) |
43.04 |
| This classifies the protein as unstable |
|
| Carbon |
2747 |
| Hydrogen |
4258 |
| Nitrogen |
738 |
| Oxygen |
794 |
| Sulfur |
21 |
| Total number of atoms |
8558 |
| Formula = C2747H4258N738O794S21 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
63995 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
63370 M-1 cm-1 |
| assuming all Cys residues are reduced |
63370 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
63995 M-1 cm-1 |
|
|
| Codon Bias |
0.054 |
| Codon Adaptation Index |
0.165 |
| Frequency of Optimal Codons |
0.451 |
| Hydropathicity of Protein |
-0.252 |
| Aromaticity Score |
0.099 |
|
