Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
49 |
7.8% |
| Arg[R] |
24 |
3.8% |
| Asn[N] |
25 |
4.0% |
| Asp[D] |
26 |
4.1% |
| Cys[C] |
16 |
2.6% |
| Gln[Q] |
13 |
2.1% |
| Glu[E] |
22 |
3.5% |
| Gly[G] |
58 |
9.3% |
| His[H] |
12 |
1.9% |
| Ile[I] |
55 |
8.8% |
| Leu[L] |
55 |
8.8% |
| Lys[K] |
25 |
4.0% |
| Met[M] |
10 |
1.6% |
| Phe[F] |
36 |
5.7% |
| Pro[P] |
26 |
4.1% |
| Ser[S] |
50 |
8.0% |
| Thr[T] |
37 |
5.9% |
| Trp[W] |
18 |
2.9% |
| Tyr[Y] |
22 |
3.5% |
| Val[V] |
48 |
7.7% |
| Total Length |
627 |
|
| Instability index (II) |
37.71 |
| This classifies the protein as stable |
|
| Carbon |
3154 |
| Hydrogen |
4836 |
| Nitrogen |
804 |
| Oxygen |
871 |
| Sulfur |
26 |
| Total number of atoms |
9691 |
| Formula = C3154H4836N804O871S26 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
132780 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
131780 M-1 cm-1 |
| assuming all Cys residues are reduced |
131780 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
132780 M-1 cm-1 |
|
|
| Codon Bias |
0.107 |
| Codon Adaptation Index |
0.110 |
| Frequency of Optimal Codons |
0.466 |
| Hydropathicity of Protein |
0.296 |
| Aromaticity Score |
0.121 |
|
