RPA190/YOR341W Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 103 6.2%
Arg[R] 79 4.7%
Asn[N] 98 5.9%
Asp[D] 105 6.3%
Cys[C] 24 1.4%
Gln[Q] 65 3.9%
Glu[E] 111 6.7%
Gly[G] 96 5.8%
His[H] 37 2.2%
Ile[I] 92 5.5%
Leu[L] 143 8.6%
Lys[K] 130 7.8%
Met[M] 43 2.6%
Phe[F] 61 3.7%
Pro[P] 64 3.8%
Ser[S] 132 7.9%
Thr[T] 104 6.2%
Trp[W] 8 0.5%
Tyr[Y] 54 3.2%
Val[V] 115 6.9%
Total Length 1664

Instability index
Instability index (II) 37.65
This classifies the protein as stable

Atomic composition
Carbon 8196
Hydrogen 13060
Nitrogen 2276
Oxygen 2550
Sulfur 67
Total number of atoms 26149
Formula = C8196H13060N2276O2550S67

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 125960 M-1 cm-1
assuming NO Cys residues appear as half cystines 124460 M-1 cm-1
assuming all Cys residues are reduced 124460 M-1 cm-1
assuming all pairs of Cys residues form cystines 125960 M-1 cm-1

Aliphatic index
Aliphatic index 81.31

Coding Region Translation Calculations
Codon Bias 0.297
Codon Adaptation Index 0.279
Frequency of Optimal Codons 0.585
Hydropathicity of Protein -0.460
Aromaticity Score 0.074