Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
68 |
7.1% |
| Arg[R] |
35 |
3.7% |
| Asn[N] |
50 |
5.2% |
| Asp[D] |
73 |
7.6% |
| Cys[C] |
7 |
0.7% |
| Gln[Q] |
26 |
2.7% |
| Glu[E] |
78 |
8.1% |
| Gly[G] |
76 |
7.9% |
| His[H] |
16 |
1.7% |
| Ile[I] |
56 |
5.8% |
| Leu[L] |
79 |
8.2% |
| Lys[K] |
91 |
9.5% |
| Met[M] |
10 |
1.0% |
| Phe[F] |
53 |
5.5% |
| Pro[P] |
38 |
4.0% |
| Ser[S] |
50 |
5.2% |
| Thr[T] |
48 |
5.0% |
| Trp[W] |
8 |
0.8% |
| Tyr[Y] |
35 |
3.7% |
| Val[V] |
61 |
6.4% |
| Total Length |
958 |
|
| Instability index (II) |
30.45 |
| This classifies the protein as stable |
|
| Carbon |
4818 |
| Hydrogen |
7496 |
| Nitrogen |
1270 |
| Oxygen |
1470 |
| Sulfur |
17 |
| Total number of atoms |
15071 |
| Formula = C4818H7496N1270O1470S17 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
96525 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
96150 M-1 cm-1 |
| assuming all Cys residues are reduced |
96150 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
96525 M-1 cm-1 |
|
|
| Codon Bias |
0.397 |
| Codon Adaptation Index |
0.378 |
| Frequency of Optimal Codons |
0.646 |
| Hydropathicity of Protein |
-0.480 |
| Aromaticity Score |
0.100 |
|
