TIM18/YOR297C Additional Physico-chemical Properties Help

Physico-chemical properties were determined using the ProtParam tool available through ExPASy to analyze the primary protein sequence for atomic composition, extinction coefficient, estimated half-life, instability index and aliphatic index. Transcript translation calculations were determined using CodonW.


A.A. Composition
Ala[A] 9 4.7%
Arg[R] 6 3.1%
Asn[N] 13 6.8%
Asp[D] 8 4.2%
Cys[C] 2 1.0%
Gln[Q] 4 2.1%
Glu[E] 8 4.2%
Gly[G] 10 5.2%
His[H] 2 1.0%
Ile[I] 14 7.3%
Leu[L] 23 12.0%
Lys[K] 14 7.3%
Met[M] 4 2.1%
Phe[F] 12 6.2%
Pro[P] 11 5.7%
Ser[S] 17 8.9%
Thr[T] 8 4.2%
Trp[W] 2 1.0%
Tyr[Y] 14 7.3%
Val[V] 11 5.7%
Total Length 192

Instability index
Instability index (II) 35.10
This classifies the protein as stable

Atomic composition
Carbon 1020
Hydrogen 1561
Nitrogen 247
Oxygen 281
Sulfur 6
Total number of atoms 3115
Formula = C1020H1561N247O281S6

Estimated half-life
Escherichia coli (in vivo) >10 hours
mammalian reticulocytes (in vitro) 30 hours
yeast (in vivo) >20 hours

Extinction Coefficients at 280 nm
assuming ALL Cys residues appear as half cystines 31985 M-1 cm-1
assuming NO Cys residues appear as half cystines 31860 M-1 cm-1
assuming all Cys residues are reduced 31860 M-1 cm-1
assuming all pairs of Cys residues form cystines 31985 M-1 cm-1

Aliphatic index
Aliphatic index 96.46

Coding Region Translation Calculations
Codon Bias 0.000
Codon Adaptation Index 0.000
Frequency of Optimal Codons 0.000
Hydropathicity of Protein 0.000
Aromaticity Score 0.000