Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
108 |
6.3% |
| Arg[R] |
102 |
6.0% |
| Asn[N] |
132 |
7.8% |
| Asp[D] |
108 |
6.3% |
| Cys[C] |
8 |
0.5% |
| Gln[Q] |
109 |
6.4% |
| Glu[E] |
129 |
7.6% |
| Gly[G] |
75 |
4.4% |
| His[H] |
37 |
2.2% |
| Ile[I] |
90 |
5.3% |
| Leu[L] |
163 |
9.6% |
| Lys[K] |
126 |
7.4% |
| Met[M] |
41 |
2.4% |
| Phe[F] |
52 |
3.1% |
| Pro[P] |
77 |
4.5% |
| Ser[S] |
118 |
6.9% |
| Thr[T] |
97 |
5.7% |
| Trp[W] |
11 |
0.6% |
| Tyr[Y] |
44 |
2.6% |
| Val[V] |
76 |
4.5% |
| Total Length |
1703 |
|
| Instability index (II) |
47.18 |
| This classifies the protein as unstable |
|
| Carbon |
8453 |
| Hydrogen |
13527 |
| Nitrogen |
2461 |
| Oxygen |
2678 |
| Sulfur |
49 |
| Total number of atoms |
27168 |
| Formula = C8453H13527N2461O2678S49 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
126560 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
126060 M-1 cm-1 |
| assuming all Cys residues are reduced |
126060 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
126560 M-1 cm-1 |
|
|
| Codon Bias |
0.088 |
| Codon Adaptation Index |
0.155 |
| Frequency of Optimal Codons |
0.457 |
| Hydropathicity of Protein |
-0.789 |
| Aromaticity Score |
0.063 |
|
