Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
47 |
7.1% |
| Arg[R] |
31 |
4.7% |
| Asn[N] |
20 |
3.0% |
| Asp[D] |
27 |
4.1% |
| Cys[C] |
5 |
0.8% |
| Gln[Q] |
13 |
2.0% |
| Glu[E] |
33 |
5.0% |
| Gly[G] |
46 |
7.0% |
| His[H] |
9 |
1.4% |
| Ile[I] |
38 |
5.8% |
| Leu[L] |
66 |
10.0% |
| Lys[K] |
32 |
4.9% |
| Met[M] |
24 |
3.6% |
| Phe[F] |
39 |
5.9% |
| Pro[P] |
43 |
6.5% |
| Ser[S] |
56 |
8.5% |
| Thr[T] |
47 |
7.1% |
| Trp[W] |
13 |
2.0% |
| Tyr[Y] |
22 |
3.3% |
| Val[V] |
48 |
7.3% |
| Total Length |
659 |
|
| Instability index (II) |
43.80 |
| This classifies the protein as unstable |
|
| Carbon |
3345 |
| Hydrogen |
5190 |
| Nitrogen |
848 |
| Oxygen |
938 |
| Sulfur |
29 |
| Total number of atoms |
10350 |
| Formula = C3345H5190N848O938S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
104530 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
104280 M-1 cm-1 |
| assuming all Cys residues are reduced |
104280 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
104530 M-1 cm-1 |
|
|
| Codon Bias |
0.185 |
| Codon Adaptation Index |
0.175 |
| Frequency of Optimal Codons |
0.502 |
| Hydropathicity of Protein |
0.078 |
| Aromaticity Score |
0.112 |
|
