Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
21 |
4.6% |
| Arg[R] |
23 |
5.0% |
| Asn[N] |
23 |
5.0% |
| Asp[D] |
37 |
8.0% |
| Cys[C] |
4 |
0.9% |
| Gln[Q] |
17 |
3.7% |
| Glu[E] |
22 |
4.8% |
| Gly[G] |
26 |
5.7% |
| His[H] |
12 |
2.6% |
| Ile[I] |
32 |
7.0% |
| Leu[L] |
41 |
8.9% |
| Lys[K] |
33 |
7.2% |
| Met[M] |
5 |
1.1% |
| Phe[F] |
10 |
2.2% |
| Pro[P] |
15 |
3.3% |
| Ser[S] |
51 |
11.1% |
| Thr[T] |
34 |
7.4% |
| Trp[W] |
7 |
1.5% |
| Tyr[Y] |
14 |
3.0% |
| Val[V] |
33 |
7.2% |
| Total Length |
460 |
|
| Instability index (II) |
38.23 |
| This classifies the protein as stable |
|
| Carbon |
2255 |
| Hydrogen |
3603 |
| Nitrogen |
633 |
| Oxygen |
718 |
| Sulfur |
9 |
| Total number of atoms |
7218 |
| Formula = C2255H3603N633O718S9 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
59610 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
59360 M-1 cm-1 |
| assuming all Cys residues are reduced |
59360 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
59610 M-1 cm-1 |
|
|
| Codon Bias |
0.212 |
| Codon Adaptation Index |
0.202 |
| Frequency of Optimal Codons |
0.536 |
| Hydropathicity of Protein |
-0.472 |
| Aromaticity Score |
0.067 |
|
