Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
24 |
3.5% |
| Arg[R] |
25 |
3.6% |
| Asn[N] |
50 |
7.3% |
| Asp[D] |
41 |
6.0% |
| Cys[C] |
12 |
1.8% |
| Gln[Q] |
25 |
3.6% |
| Glu[E] |
52 |
7.6% |
| Gly[G] |
18 |
2.6% |
| His[H] |
10 |
1.5% |
| Ile[I] |
59 |
8.6% |
| Leu[L] |
86 |
12.6% |
| Lys[K] |
46 |
6.7% |
| Met[M] |
18 |
2.6% |
| Phe[F] |
35 |
5.1% |
| Pro[P] |
17 |
2.5% |
| Ser[S] |
63 |
9.2% |
| Thr[T] |
34 |
5.0% |
| Trp[W] |
7 |
1.0% |
| Tyr[Y] |
27 |
3.9% |
| Val[V] |
36 |
5.3% |
| Total Length |
685 |
|
| Instability index (II) |
41.54 |
| This classifies the protein as unstable |
|
| Carbon |
3564 |
| Hydrogen |
5608 |
| Nitrogen |
908 |
| Oxygen |
1071 |
| Sulfur |
30 |
| Total number of atoms |
11181 |
| Formula = C3564H5608N908O1071S30 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
79480 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
78730 M-1 cm-1 |
| assuming all Cys residues are reduced |
78730 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
79480 M-1 cm-1 |
|
|
| Codon Bias |
0.001 |
| Codon Adaptation Index |
0.123 |
| Frequency of Optimal Codons |
0.421 |
| Hydropathicity of Protein |
-0.165 |
| Aromaticity Score |
0.101 |
|
