Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
22 |
4.3% |
| Arg[R] |
22 |
4.3% |
| Asn[N] |
34 |
6.7% |
| Asp[D] |
24 |
4.7% |
| Cys[C] |
7 |
1.4% |
| Gln[Q] |
17 |
3.3% |
| Glu[E] |
31 |
6.1% |
| Gly[G] |
27 |
5.3% |
| His[H] |
7 |
1.4% |
| Ile[I] |
30 |
5.9% |
| Leu[L] |
49 |
9.6% |
| Lys[K] |
37 |
7.3% |
| Met[M] |
13 |
2.6% |
| Phe[F] |
18 |
3.5% |
| Pro[P] |
31 |
6.1% |
| Ser[S] |
69 |
13.6% |
| Thr[T] |
26 |
5.1% |
| Trp[W] |
6 |
1.2% |
| Tyr[Y] |
17 |
3.3% |
| Val[V] |
21 |
4.1% |
| Total Length |
508 |
|
| Instability index (II) |
48.54 |
| This classifies the protein as unstable |
|
| Carbon |
2500 |
| Hydrogen |
3956 |
| Nitrogen |
682 |
| Oxygen |
782 |
| Sulfur |
20 |
| Total number of atoms |
7940 |
| Formula = C2500H3956N682O782S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
58705 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
58330 M-1 cm-1 |
| assuming all Cys residues are reduced |
58330 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
58705 M-1 cm-1 |
|
|
| Codon Bias |
-0.021 |
| Codon Adaptation Index |
0.105 |
| Frequency of Optimal Codons |
0.384 |
| Hydropathicity of Protein |
-0.505 |
| Aromaticity Score |
0.081 |
|
