Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
34 |
8.0% |
| Arg[R] |
16 |
3.8% |
| Asn[N] |
27 |
6.4% |
| Asp[D] |
29 |
6.9% |
| Cys[C] |
9 |
2.1% |
| Gln[Q] |
21 |
5.0% |
| Glu[E] |
15 |
3.5% |
| Gly[G] |
25 |
5.9% |
| His[H] |
8 |
1.9% |
| Ile[I] |
29 |
6.9% |
| Leu[L] |
28 |
6.6% |
| Lys[K] |
22 |
5.2% |
| Met[M] |
11 |
2.6% |
| Phe[F] |
12 |
2.8% |
| Pro[P] |
13 |
3.1% |
| Ser[S] |
54 |
12.8% |
| Thr[T] |
23 |
5.4% |
| Trp[W] |
8 |
1.9% |
| Tyr[Y] |
16 |
3.8% |
| Val[V] |
23 |
5.4% |
| Total Length |
423 |
|
| Instability index (II) |
52.31 |
| This classifies the protein as unstable |
|
| Carbon |
2030 |
| Hydrogen |
3171 |
| Nitrogen |
565 |
| Oxygen |
653 |
| Sulfur |
20 |
| Total number of atoms |
6439 |
| Formula = C2030H3171N565O653S20 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
68340 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
67840 M-1 cm-1 |
| assuming all Cys residues are reduced |
67840 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
68340 M-1 cm-1 |
|
|
| Codon Bias |
0.020 |
| Codon Adaptation Index |
0.128 |
| Frequency of Optimal Codons |
0.421 |
| Hydropathicity of Protein |
-0.359 |
| Aromaticity Score |
0.085 |
|
