Physico-chemical properties were determined using the
ProtParam tool available
through ExPASy to analyze the
primary protein sequence for atomic composition, extinction coefficient,
estimated half-life, instability index and aliphatic index. Transcript
translation calculations were determined using
CodonW.
| Ala[A] |
48 |
5.4% |
| Arg[R] |
39 |
4.4% |
| Asn[N] |
47 |
5.3% |
| Asp[D] |
59 |
6.7% |
| Cys[C] |
7 |
0.8% |
| Gln[Q] |
27 |
3.1% |
| Glu[E] |
63 |
7.2% |
| Gly[G] |
37 |
4.2% |
| His[H] |
18 |
2.0% |
| Ile[I] |
60 |
6.8% |
| Leu[L] |
109 |
12.4% |
| Lys[K] |
83 |
9.4% |
| Met[M] |
22 |
2.5% |
| Phe[F] |
27 |
3.1% |
| Pro[P] |
30 |
3.4% |
| Ser[S] |
89 |
10.1% |
| Thr[T] |
45 |
5.1% |
| Trp[W] |
3 |
0.3% |
| Tyr[Y] |
23 |
2.6% |
| Val[V] |
45 |
5.1% |
| Total Length |
881 |
|
| Instability index (II) |
40.80 |
| This classifies the protein as unstable |
|
| Carbon |
4382 |
| Hydrogen |
7124 |
| Nitrogen |
1194 |
| Oxygen |
1357 |
| Sulfur |
29 |
| Total number of atoms |
14086 |
| Formula = C4382H7124N1194O1357S29 |
|
| Escherichia coli (in vivo) |
>10 hours |
| mammalian reticulocytes (in vitro) |
30 hours |
| yeast (in vivo) |
>20 hours |
|
| assuming ALL Cys residues appear as half cystines |
51145 M-1 cm-1 |
| assuming NO Cys residues appear as half cystines |
50770 M-1 cm-1 |
| assuming all Cys residues are reduced |
50770 M-1 cm-1 |
| assuming all pairs of Cys residues form cystines |
51145 M-1 cm-1 |
|
|
| Codon Bias |
0.057 |
| Codon Adaptation Index |
0.150 |
| Frequency of Optimal Codons |
0.437 |
| Hydropathicity of Protein |
-0.393 |
| Aromaticity Score |
0.060 |
|
